Molecular Analysis of Topoisomerase IV Mutation in Ciprofloxacin Resistant Clinical Strains of Shigella spp. in Bushehr

Madineh Zendehboodi, Hadi Rezaei, Mohammad Ali Haghighi, Somayyeh Gharibi, Mahnaz Gholipour-Shahraki ©

Molecular Analysis of Topoisomerase IV Mutation in Ciprofloxacin Resistant Clinical Strains of Shigella spp. in Bushehr

Code: G-05964

Authors: Madineh Zendehboodi, Hadi Rezaei, Mohammad Ali Haghighi, Somayyeh Gharibi, Mahnaz Gholipour-Shahraki ©

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Abstract: Background: Shigella spp. cause acute diarrhea that may lead to the death of patients, especially children under 5 in developing countries. Ciprofloxacin, as the first line treatment for shigellosis, inhibit the growth of bacteria trough the interruption in the activity of topoisomerase IV and DNA gyrase enzymes. Bacteria use different mechanisms to resist which may resulted in the failing of antibiotic treatments. So far, chromosomal mutations in target genes that led to amino acid replacement in topoisomerase IV subunits, ParC and ParE, are one of the most important mechanism. This study aims to analysis of the mutation in parC and parE in clinical isolates of ciprofloxacin resistant Shigella spp. in Bushehr city. Material and Methods: The nucleotide mutations in parC and parE genes was investigated in 5 resistant and 5 semi-sensitive strains of Shigella spp. through amplification of the target genes by PCR and nucleotide sequencing. Results: The results indicated there was a mutation in the parC gene (Ser80Ile) in 60% of ciprofloxacin-resistant strains. Mutations in the parE gene (Asp408Gly) were also reported in 80% of resistant strains and 20% of semi-sensitive strains. Conclusion: The results of this study showed that mutations in parC and parE genes in Shigella spp. clinical isolates play an important role in ciprofloxacin resistance. Mutation patterns in both genes were similar to other studies, and that may reflect the global pattern of resistance. However, more research is needed to examine other mechanisms involved in ciprofloxacin resistance. Key Words: Shigella spp., Ciprofloxacin, PCR, Sequencing

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