تایپینگ ژن پروتئین M استرپتوکوکوس پیوژنز مرتبط با فارنژیت و سپتی سمی در بوشهر
نویسندگان: Somayyeh gharibi ©, Mahnaz Gholipour shahraki, Hengameh Abdoli, Abbas Yadegar, Saeed Tajbakhsh, Zeinab Asen
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خلاصه مقاله: Background: Streptococcus pyogenes (GAS) continues to be a significant cause of suppurative and nonsuppurative human infections throughout the world. Accurate identification of group A streptococci is an essential tool in studies of the epidemiology, pathogenesis, and therapy of streptococcal infections. Demonstration that M protein is an important Streptococcus pyogenes virulence factor and that antibody to this protein confers protection to the human host led to the acceptance of M typing as the preferred method for GAS characterization throughout most of the twentieth century. Advances in DNA-sequencing technology in the late twentieth century resulted in the development of a method for determining the M type of GAS from the sequence of the gene encoding M protein, the emm gene. Therefore, the N-terminal sequencing of the emm gene has replaced older methods, and more than 200 emm types have been identified worldwide. Therefore, the aim of this study was to investigate M protein typing associated with pharyngitis and sepsis by PCR.
Material and Methods: After DNA extraction and amplification of 4 strains emm gene isolated from patients with pharyngitis (3 samples) and sepsis (1 sample), the PCR products was sent to Topaz Gene Company for sequencing by maintaining the cold chain. DNA of Streptococcus pyogenes ATCC 19615 was used as positive control. Using the BLAST program, which is available at www.ncbi.nlm.nih.gov/BLAST, gene sequences were compared and typed with sequences published in the gene bank.
Results: Three different emm types were identified. The most common of these was emm89. emm76, and emm80 were identified in one clinical strain and ATCC strain, respectively.
Conclution: The results showed the presence of different types of emm isolated from pharyngitis and septicemia compared to isolates in other parts of the world. However, due to the low statistical population, it is better to sequence more strains for more detailed study.
Key Words: emm, M typing, Streptococcus pyogenes GAS, Pharyngites, Septicemia, PCR
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