Evaluation of the Effect of Biosynthetic Nanoparticles of Copper Oxide on AmpC Gene Expression in Clinical Strains of Pseudomonas Aeruginosa
نویسندگان: Hossein Azizi, Farnaz Kheirandish ©, Neda Akbari, Asghar Sepahvand
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Background and Aim
Nowadays, the need for new strategies to control the activities of resistant bacteria is felt more than ever. In this regard, a very promising approach is the use of nanomaterials science and the knowledge that is expanding in the field of nano synthesis biosynthesis should be considered as a global strategy. The aim of this study was to evaluate the antibacterial properties of biosynthetic copper oxide nanoparticles on the expression of the AmpC gene in Pseudomonas aeruginosa.
Ten samples of P. aeruginosa were collected from clinical centers of Khorramabad city in 1399 and identified based on biochemical tests. After treatment of MDR strains with the minimum inhibitory concentration of copper oxide nanoparticles, the expression level of the AmpC gene was evaluated using real-time PCR.
After applying the effect of biosynthetic copper oxide nanoparticles on AmpC gene expression and calculations performed by MIC and ANOVA one-Tukey statistical analysis test, it was found that copper oxide nanoparticles have an inhibitory effect on the expression of AmpC genes of P. aeruginosa samples.
The objectives of this study focused on the use of nanotechnology as a new tool to address current challenges in the treatment of infectious diseases and antibiotic resistance. Therefore, for safer use of this nascent technology, the need for clinical trials and further research at the level of molecular biology is recommended.
Nanoparticle; Multidrug Resistance; Real-time PCR; Pseudomonas aeruginosa
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