Current trends in colistin susceptibility testing methods in enteric Gram-negative bacilli and other non-fermentative pathogens

Khadijeh Najafi, Leila Yousefi, Elham Zeinalzadeh, Hossein Samadi Kafil ©

Current trends in colistin susceptibility testing methods in enteric Gram-negative bacilli and other non-fermentative pathogens

کد: G-34517

نویسندگان: Khadijeh Najafi, Leila Yousefi, Elham Zeinalzadeh, Hossein Samadi Kafil ©

زمان بندی: زمان بندی نشده!

برچسب: باکتری شناسی پزشکی

دانلود: دانلود پوستر

خلاصه مقاله:

Background and Aim

Polymyxins, an age-old sort of cationic polypeptide antibiotic, are regarded as final-resort drugs in handling infections caused by multidrug-resistant Gram-negative bacteria. An exact assessment of colistin susceptibility is crucial due to increasing colistin resistance and absence of other antibiotics in curing multidrug resistance pathogens. Both Clinical and Laboratory Standards Institute and European Committee on Antimicrobial Susceptibility Testing have investigated colistin broth microdilution and offer particular recommendations, but this method has a challenging perform in routine daily practice. It is time consuming and requires ≥48 h. In this issue, several alternative rapid tests are developed such as ResaPolymyxin Acinetobacter/Pseudomonas NP. The sensitivity and specificity of 100% for the Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test to Enterobacterales have been reported. Colistin broth disk elution test and colistin agar test are another novel potential methods that were validated by the Clinical laboratory standard institutes subcommittee for colistin screening in the Enterobacterales and Pseudomonas aeruginosa. In addition, recently, Rapid flow cytometry (FASTinov) method was introduced which requires only 2 h compared with conventional methods. This review will take a look at current trends on methods for colistin resistance measurements.

Methods

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Results

Nowadays, both EUCAST and CLSI subcommittee awarded suggestion verifying that broth microdilution (BMD) has been until now the only applicable method. Disk diffusion does not work because of the poor diffusion of the large colistin molecule. E-test is unreliable by giving warning from EUCAST and other studies.

Conclusion

The review of the included articles has revealed that the emergence of plasmid-mediated (mcr genes) and chromosomally encoded colistin resistance is considered a serious problem due to a lack of alternative antibiotics and reliable methods for detecting colistin resistance and susceptibility in routine laboratories are needed, this is not only critical for optimum susceptibility testing, but also helps to guide decision-making for patient treatment.

Keywords

Gram negative bacteria; antimicrobial susceptibility testing; colistin; invitro activity; method

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