Anti-protozoal activity of magnesium oxide (MgO) and calcium oxide (CaO) nanoparticles againstLeishmania major In vitro
نویسندگان: Mohammad Barati ©, Ali Dalir Ghaffari
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انگل شناسی پزشکی
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Background and Aim
Leishmaniasis is a parasitic infection that is more common in the tropical and subtropical regions.The pentavalent antimonial compounds are the first drug of choice for Leishmania infection, but have several side effects that cause some restriction for use. The current study was aimed to evaluate the effect of magnesium oxide (MgO) and calcium oxide (CaO) Leishmania major in-vitro.
To evaluate the antileishmanial activity of nanoparticles (NPs), light microscopic observation was used to determine the number of remaining parasites in each well. In this study, L. major promastigotes were exposed to MgO and Cao NPs with different doses ranging from 2.5-40 mg/ml. Then MTT test was used to determine the cytotoxicity effects of MgO and CaO NPs against L. major. Moreover, lethal effects of nanoparticles were tested for macrophage cell with MTT assay.
At high concentrations, two nanoparticles had a more lethal effect on parasites. According to the results of MTT assay, the nanoparticles via MgO and CaO NPs led to the death of parasite cells. High concentrations of nanoparticles led to the destruction of macrophages so that less of 20%.
Based on the above assessment, we determined that the Mgo NP has a more lethal effect on promastigotesthan CaO NP and maybe in the future that be used as a drug candidate.
Leishmania major; Magnesium oxide; Calcium oxide; In-vitro
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