Expression evaluation of APOBEC3B, COL1A1, and HLF genes in HNSCC

Mohsen Ahmadi, Parisa Najari Hanjani, Negin Saffarzadeh ©, Pegah Mousavi

Expression evaluation of APOBEC3B, COL1A1, and HLF genes in HNSCC

کد: G-64359

نویسندگان: Mohsen Ahmadi, Parisa Najari Hanjani, Negin Saffarzadeh ©, Pegah Mousavi

زمان بندی: زمان بندی نشده!

برچسب: ژنتیک پزشکی

دانلود: دانلود پوستر

خلاصه مقاله:

Background and Aim

Squamous cell carcinoma of the head and neck has been identified as the sixth most common cancer worldwide. APOBEC3B may be involved in cancer development because of its ability to increase genomic instability. COL1A1 is closely linked to tumors and other diseases and can regulate multiple cancers by acting as an oncogene or tumor suppressor. Some studies have suggested a vital role for HLF in regulating certain types of cancer. This study aimed to measure the relative expression of three genes (APOBEC3B, HLF, and COL1A1) in tumor tissue and healthy adjacent samples with head and neck squamous cell carcinoma and to determine their potential capacity for utilizing them as a diagnostic marker.

Methods

Processed expression data (RNA-seq) and clinicopathological data of tumor tissues of 520 patients with HNSCC and 44 healthy individuals from TCGA-HNSCC cohort were achieved for bioinformatics study and then 45 fresh tumor samples of head and neck squamous cell carcinoma and 45 healthy adjacent samples were collected. Then, RNA extraction and cDNA synthesis was conducted. Finally, the relative expression of three genes (APOBEC3B, HLF, and COL1A1) was measured by Real-Time PCR.

Results

Our findings disclosed that while APOBEC3B and COL1A1 were significantly upregulated, and the expression of HLF was decreases in HNSCC tissues comparing to normal ones. Moreover, the area under curve value for APOBEC3B, COL1A1, and HLF genes were 0/747, 0/861, and 0/767, respectively, and in a combination manner, as a three-gene panel was 0/982.

Conclusion

Our research was the first to investigate the expression of the APOBEC3B and COL1A1 genes in Iran and HLF worldwide in HNSCC. Besides, the acquired panel showed a promising diagnostic value for HNSCC.

Keywords

Head and neck squamous cell carcinoma; APOBEC3B; COL1A1; HLF

دیدگاه ها (0)

تاکنون دیدگاهی منتشر نشده است. شما اولین نفر باشید!

ارسال یک دیدگاه

ارسال دیدگاه توسط مدیریت بسته شده است.