The Expression Level of CCDC26 and FOXCUT Genes in Acute Lymphoblastic Leukemia

Seyed Hadi Mousavi ©, Samaneh Barati, Jamal Motallebzadeh Khanmiri, Mohammad Khani-Eshratabadi

The Expression Level of CCDC26 and FOXCUT Genes in Acute Lymphoblastic Leukemia

کد: G-84695

نویسندگان: Seyed Hadi Mousavi ©, Samaneh Barati, Jamal Motallebzadeh Khanmiri, Mohammad Khani-Eshratabadi

زمان بندی: زمان بندی نشده!

برچسب: هماتولوژی

دانلود: دانلود پوستر

خلاصه مقاله:

Background and Aim

Background and Aim: Acute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid progenitor cells affecting both children and adults. Long non-coding RNAs (lncRNAs) are one kind of non-coding RNAs (ncRNAs), reported modulating the initiation or progression of diverse cancers. However, the role of CCDC26 and FOXCUT long non-coding RNAs in ALL has been unknown. In this study, we explored the expression of FOXCUT and CCDC26 lncRNAs in acute lymphoblastic leukemia cell lines.

Methods

Methods: Acute T lymphoblastic leukemia cell lines, RPMI 8402, Jurkat, B lymphoblastic leukemia, Daudi, and Ramos cell lines were used. After culturing the cells, RNA extraction and cDNA synthesis were performed. The real-time PCR technique was then used to study the expression of CCDC26, FOXCUT, C-kit, and FOXC1 genes.

Results

We found a significant increase of CCDC26 expression in RPMI 8402 ( p-value is less than 0.0001) and Ramos ( p-value is less than 0.05) cell lines compared to the control, while decreased expression of these genes was observed in Jurkat and Daudi cell lines. Furthermore, FOXCUT gene had a significant increase in expression in all cell lines compared to the control ( p-value is less than 0.01 in Daudi and RPMI 8402 cell lines) ( p-value is less than 0.001 in Jurkat and Ramos cell lines).

Conclusion

Our results demonstrated that CCDC26 and FOXCUT genes can play a regulatory role in acute lymphoblastic leukemia and may serve as a potential diagnostic biomarker and therapeutic target of acute lymphoblastic leukemia.

Keywords

Acute Lymphoblastic; Leukemia; Long Non-Coding RNA CCDC26 C-Kit FOXCUT

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