Evaluation of Varicella-Zoster virus in leukemias by PCR molecular method
کد: G-10463
نویسندگان: Taraneh Rahenow ©, Mohammad Hassan Shahhosseiny, Taher Mohammadian, Atousa Ferdosi
زمان بندی: زمان بندی نشده!
برچسب: ویروس شناسی پزشکی
دانلود: دانلود پوستر
خلاصه مقاله:
Background and Aim
Leukemia begins with the atrophy of bone marrow and progresses through the destruction of myeloid or lymphoid lineages over time. In immunocompromised patients and children with leukemia, there is a potential for Varicella-Zoster virus. In this study, among the DNA-based methods, PCR has been used to evaluate the DNA of Varicella-Zoster virus in the serum of patients with leukemia, which is one of the most accurate methods approved by the WHO.
Methods
The PCR test was optimized for VZV detection and was evaluated in terms of specificity and limit of detection (LOD). The DNA was extracted from 100 serum specimens of patients with leukemia and 100 healthy control specimens (positive and negative) from Tehran laboratories by phenol-chloroform. The product of PCR was detected using Agarose gel electrophoresis method.
Results
The varicella-zoster virus didn’t observe in all healthy controls, but it was seen in nine percent (9%) of patients’ specimens. The PCR test was optimized and 216-bp product was observed in agarose gel electrophoresis (Fig 1). In specificity test, there was no product other than the VZV specimen(Fig 2). A detection limit of 10 Copy / Reaction was reported (Fig 3).
Conclusion
In leukemia, besides environmental and genetic factors, infectious agents such as some viruses are effective. According to this study, VZV, after several periods of proliferation and accumulation in the skin, can be considered as an effective infectious agent in leukemia due to its severe pathogenicity.
Keywords
Varicella-Zoster virus; PCR; Leukemia
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