Human T-cell leukemia virus type 1 (HTLV-1) which cause two serious diseases in human and proviral load (PVL) of virus can predict the progression of disease. Programmed cell death-1 (PD-1) is a regulatory protein that created exhaustion phenotype in T cells and influence in immune system response during viral infection. In this research, association between rs10204525 and rs2227981 polymorphisms, in exon 5 of PD-1 gene, with susceptibility to HTLV-1 infection and PVL in Iran’s population studied.
In this case-control study, PD-1 rs10204525 and rs2227981polymorphisms were evaluated in 162 healthy individuals and 81 HTLV-1 asymptomatic carriers (ACs). These polymorphisms were genotyped by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PVL was detected by quantitative real-time PCR (Q-RT-PCR).
Frequency of GA and AA genotypes in rs10204525 polymorphism were higher in ACs group (82.7%) than control group (26.5%) significantly; and GA + AA genotypes were significantly associated with HTLV-1 infection (OR = 13.244, 95%CI = 6.755–25.968, p = 0.000); but CT + TT genotypes in rs2227981 polymorphism, were as a protective factor against HTLV-1 infection (OR = 0.473, 95%CI = 0.279–0.813, P = 0.009). However, there was no significant difference between these polymorphisms and HTLV-1 PVL.
The results indicated that there is an association between rs10204525 and rs2227981 polymorphisms with susceptibility to HTLV-1 infection in Iran’s population.