Prevalence of enteropathogenic Escherichia coli in mucosal samples of patients with colorectal cancer and control subjects
کد: G-73162
نویسندگان: Roghayeh Nouri, Mohammad Ahangarzadeh Rezaee ©, Alka Hasani, Kourosh Masnadi Shirazi, Mohammad Reza Alivand, Bita Sepehri, Faezeh Ahmadzadeh
زمان بندی: زمان بندی نشده!
برچسب: باکتری شناسی پزشکی
دانلود: دانلود پوستر
خلاصه مقاله:
Background and Aim
Enteropathogenic Escherichia coli (EPEC) is an attaching and effacing (A/E) pathogen that causes diarrhea in acute infection. But, recent studies have shown that some strains of EPEC are able to survive and replicate in colonic epithelial cells as a chronic intracellular pathogen and subvert some host cell functions ultimately leading to colorectal cancer (CRC). The aim of this study was to compare the prevalence of EPEC among mucosa-associated E. coli strains from CRC patients and healthy controls.
Methods
From August 2019 to June 2020, 45 CRC patients and 45 non-cancerous subjects from two referral university-affiliated hospitals in northwest Iran were enrolled in this study. The biopsy specimens were obtained from the colon and rectum was used to identify mucosa-associated E. coli isolates. The boiling method was used to extract DNA from E.coli strains. The PCR method was used to detect for the presence of the eae, stx1, stx2 and bfp genes.
Results
Typical EPEC is defined as strains possessing eae and bfp genes but no stx1, stx2 genes. In addition to stx1, stx2 genes, atypical strains of EPEC also lack the bfp gene. In this study the eae gene was detected in 24.4% of E. coli strains from CRC patients, but only in 6.6% of strains from control group (p < 0.05). Moreover, all strains possessed eae gene were negative for bfp stx1, stx2 and genes. Therefore, all EPEC strains were atypical EPEC.
Conclusion
The gut mucosa of CRC patients provides suitable conditions for colonization of EPEC, and these strains may play a role in the pathogenesis of CRC.
Keywords
Colorectal cancer; Enteropathogenic; Escherichia coli; PCR
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