Allergenicity and immunochemical characterization of five common regional plant pollens

Sajjad Chamani, Mohammad Fereidouni ©

Allergenicity and immunochemical characterization of five common regional plant pollens

کد: G-35748

نویسندگان: Sajjad Chamani, Mohammad Fereidouni ©

زمان بندی: زمان بندی نشده!

برچسب: ایمنی شناسی

دانلود: دانلود پوستر

خلاصه مقاله:

Background and Aim

Background: The inhalation of plants pollen grains is one of the most important triggers of allergic symptoms which has a high impact on patients’ quality of life as well as a high important economic burden. Knowing the main source of allergenic pollens in each area is the first step in the prevention and management of inhalant allergic disorders. Objective: This study aimed to evaluate the allergenicity and immunochemical characterization of five common regional plant pollens

Methods

Methods: Pure pollen of five abundant plants in the area, including Eucalyptus tree (blue gum), Rosa damascene flowers (Damask rose), Jasminum sp flowers (Persian Jasmine), Phoenix dactylifera tree (palm), and Pistacia vera trees (pistachio), were collected during pollination season and then aqueous extracts were prepared. After receiving ethics committee approval, the five extracts, as well as several commercial allergenic extracts, were tested on thirty-seven people with a history of allergic rhinitis, by the skin prick test method. The protein profile of domestic extracts was evaluated by SDS-PAGE and, the allergenic profile was investigated by immunoblotting using pooled serum collected from sensitive patients.

Results

Results: The highest and lowest prevalence of skin sensitivity to domestic extracts was for pollens of the Eucalyptus tree (59.45%) and Phoenix dactylifera tree. The largest mean wheal size was for Rosa damascene flowers and, Eucalyptus tree. The antigenic and allergenic profiles of the extracts also varied. The resolved protein fractions on SDS-PAGE ranged from 5–85 kDa. There were several reactive bands for each extract but the main reactive band for each extract was as follows: Eucalyptus 55 kDa, Jasminum sp 45 kDa, Phoenix dactylifera 40 kDa, Rosa damascene 30 kDa and, Pistacia vera 25 kDa.

Conclusion

Conclusion: In conclusion, the analysis demonstrated these five plants as the aeroallergens which could cause IgE induced hypersensitivity reaction

Keywords

Allergens; Allergic rhinitis; IgE; Immunoblotting; Pollen; SDS-PAGE

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