First molecular report of causative agent of otomycosis due to Aspergillus luchuensis

Hasti Kamali Sarvestani ©, Roshanak Daie Ghazvini, Mohsen Gerami Shoar, Shahram Mahmoudi, Miad Elahi, Mohammad Taghi Hedayati, Seyed Jamal Hashemi

First molecular report of causative agent of otomycosis due to Aspergillus luchuensis

کد: G-51790

نویسندگان: Hasti Kamali Sarvestani ©, Roshanak Daie Ghazvini, Mohsen Gerami Shoar, Shahram Mahmoudi, Miad Elahi, Mohammad Taghi Hedayati, Seyed Jamal Hashemi

زمان بندی: زمان بندی نشده!

برچسب: قارچ شناسی پزشکی

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خلاصه مقاله:

Background and Aim

Otomycosis is a fungal infection of the external auditory canal with worldwide distribution. It is often reported from hot, humid, and dusty areas of tropical and subtropical regions. Among a broad spectrum of saprophytic filamentous fungi, yeasts, and dermatophytes, Aspergillus was reported as the most common genus responsible for otomycosis. Aspergillus currently includes 25 sections. The most common cause of otomycosis was reported as Aspergillus section Nigri (black aspergilli). Section Nigri comprises 27 species of which 11 were reported from different human clinical entities, including otomycosis, pulmonary aspergillosis, aspergilloma, and onychomycosis. Also, Aspergillus section Nigri, including Aspergillus niger and other cryptic species, has received increasing attention worldwide because of their role in industrial exploitation, prevention of food spoilage, and toxin production. To our knowledge, in this case, we report the first clinically relevant isolation of Aspergillus luchuensis identified by molecular technique as a causative agent of otomycosis.

Methods

A 43-year-old female patient with a left ear infection was admitted to a specialty ear, nose and throat (ENT) hospital (Amir Alam Hospital of Tehran University of Medical Sciences (TUMS), Tehran, Iran). The clinical presentations included otalgia (pain in the ear), otorrhoea (purulent discharge from the ear), itching and the otorrhoea had persisted for one month while she remained afebrile. On otoscopic examination, an eclipse was seen that occupied her left external ear canal. The audiogram showed a hearing loss in the left ear. The patient had a history of frequent swimming, the use of cotton swab and seasonal allergy. Otoscopic examination revealed inflammation in the external auditory canal. Debris samples were collected from the outer ear with aseptic precautions by sterile cotton swabs and sterile forceps. Collected samples underwent microscopic examination and culture immediately. Direct examination of the specimen, using 10% potassium hydroxide, revealed septate hyphae and fruiting bodies consistent with Aspergillus species. The specimen's culture onto sabouraud dextrose agar (SDA, Merck, Germany) plates yielded growth of colonies compatible with Aspergillus section Nigri. The amplicons were sequenced (Macrogen, South Korea), and the results were visually checked and compared with the GenBank sequences using nucleotide blast analysis (https://www.blast.ncbi.nlm.nih.gov/Blast.cgi).

Results

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Conclusion

Besides the necessity for accurate identification, evaluating susceptibility profiles of clinical Aspergillus isolates is also needed. This would support treatment, as well as enhance understanding of the epidemiology of Aspergillus species.

Keywords

Aspergillus; Section nigri; molecular; antifungal; otomycosis

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