Frequency of Legionella pneumophila virulence genes in hospital water supply systems of western Iran
نویسندگان: Manouchehr Ahmadi Hedayati ©
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Background and Aim
Legionella pneumophila is still a common cause of Hospital-acquired pneumonia. In this regard, hospital water supply systems are an important resource of bacterial transfer. The aim of this study was to detect L. pneumophila in water supply systems of Sanandaj֝s hospitals.
In a cross-sectional study, 100 water samples from hospital water supply systems including shower of bathrooms, laboratory water bath, water cooler tank, and ponds were collected during 6 months, from February to November in 2020. The L. pneumophila were examined through culture on BCYE and Hippurate hydrolysis test. Then PCR was used as molecular method by using Legionella species primers.
twelve strains of Legionella species were isolated from the water samples through culture on BCYE medium. Furthermore, the prevalence of 16s rRNA, mip and rtxA genes detected in water samples by PCR were 16%, 4% and 4%, respectively. 4 strains of Legionella isolated were determined L. pneumophila through hippurate hydrolysis test.
A considerable prevalence of mip and rtxA genes was detected in environmental strains of L. pneumophila isolated from Sanandaj֝s hospital water supply systems.
Legionella pneumophila; 16s rRNA; mip; rtxA; PCR; Hospital Water resources
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