Evaluate the effect of mesenchymal stem cells derived exosomes on regulatory T-cell function in COVID‐19 patients PBMCs

Ali Hazrati, Majid Ahmadi ©, Kosar Malekpour

Evaluate the effect of mesenchymal stem cells derived exosomes on regulatory T-cell function in COVID‐19 patients PBMCs

کد: G-84512

نویسندگان: Ali Hazrati, Majid Ahmadi ©, Kosar Malekpour

زمان بندی: زمان بندی نشده!

برچسب: ایمنی شناسی

دانلود: دانلود پوستر

خلاصه مقاله:

Background and Aim

During the COVID-19 pandemic, much attention was paid to the function of immune cells in the pathogenesis of the disease. During the cytokine storm, which is part of the pathogenesis of this disease, a large amount of inflammatory cytokine is produced, leading to the occurrence of acute respiratory syndrome. Regulatory t cells suppress the immune system cell responses by producing inhibitory cytokines such as interleukin-10 (IL-10) and transforming growth factor-B (TGF-B) and cell-to-cell interaction. Therefore, studying the function of regulatory T cells in patients with Covid-19 is of particular importance and can help to better understand the pathogenesis and find treatments based on the increase of regulatory T cells. Since mesenchymal stem cells-derived exosomes have immunomodulatory properties, the use of these vesicles can improve the function of Tregs in COVID-19 patient PBMCs.

Methods

In this study, mesenchymal stem cells were first isolated from adipose tissue. After culturing these cells, the exosome was isolated from their supernatant by ultracentrifugation. SEM, TEM and DLS confirm isolated exosomes characterization. Then PBMCs isolated from patients with COVID-19 cultured in the presence of these exosomes. The expression of transcription factor related to these cells and the secretion levels of cytokines was assessed by real-time PCR and ELISA, respectively, before and after treatment with MSCs exosomes.

Results

A significant reduction was observed in the levels of TGF-β, IL-10, and FoxP3 expression in COVID-19 patient PBMCs. Our data revealed the expression of FoxP3, TGF-β, and IL-10, the secretion levels of the TGF-β, and IL-10 in cultured PBMCs are increased in MSCs-derived exosome-treated group compared to the control group.

Conclusion

The current study results indicated that AD-MSCs derived exosomes can restore the function of Treg cells of COVID-19 patient isolated PBMCs.

Keywords

MSCs; Exosome; PBMCs; COVID-19; Cytokine

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