Detection of GES and VEB extended spectrum β-lactamase in Klebsiella oxytoca in khorramabad, Iran

Gholamreza Goudarzi ©, Pegah Shakib

Detection of GES and VEB extended spectrum β-lactamase in Klebsiella oxytoca in khorramabad, Iran

کد: G-04983

نویسندگان: Gholamreza Goudarzi ©, Pegah Shakib

زمان بندی: زمان بندی نشده!

برچسب: باکتری شناسی پزشکی

دانلود: دانلود پوستر

خلاصه مقاله:

Background and Aim

Klebsiella oxytoca is an immobilized gram-negative bacillus, aerobic, voluntary anaerobic, oxidase negative, belongs to the Enterobacteriaceae family and is considered as an opportunistic pathogen. Klebsiella oxytoca like other members of the Enterobacteriaceae family, may acquire genes encoding resistance, including extended spectrum beta-lactamases (ESBL), metallo-β-lactamases, and carbapenemases. The aim of this study was detection of VEB and GES genes in Klebsiella oxytoca isolated from clinical specimens in khorramabad, Iran.

Methods

Klebsiella oxytoca collected from hospitals of Khorramabad, Iran from January 2019 to January 2020.Then ESBL-producing strains were isolated by disc diffusion method according to CLSI protocol. After DNA extraction from all Klebsiella oxytoca clinical strains VEB and GES genes detected by PCR method.

Results

The present study was a cross-sectional study, 32 Klebsiella oxytoca were collected randomly. Of these, 21 strains (65.6%) were isolated from women and 11 strains (34.4%) were isolated from men. The majority of people were sampled in the age group (41-60). The mean age of the subjects was 22.18 ± 46.16 years. Most strains were isolated from urine samples with frequency of 87.5% and then from wounds and sputum with a frequency of 6.3% each. Among 32 isolates, 56.6% (N = 21) of isolates were ESBL positive. Also, the results showed that none of isolates were carried VEB and GES genes.

Conclusion

The results of the present study indicate the spread of ESBLs and the absence of VEB and GES genes, which requires more extensive studies to identify other ESBL genes.

Keywords

Extended Spectrum Β-Lactamase (ESBL); GES; VEB; PCR

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