Human Respiratory Syncytial Virus in children less than 5 years old hospitalized in Tabriz Childrens Hospital
نویسندگان: Mahin Ahangar Oskouee ©, Zahra Ramezannia, Arezou Azadi, Rana Farzi
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ویروس شناسی پزشکی
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Background and Aim
Acute respiratory tract infection (ARTI) is a common reason for hospitalization in children that continues to threaten public health. ARTI is commonly caused by viruses, including human respiratory syncytial virus (HRSV), human Parainfluenza virus (HPIV), human bocavirus virus (HBoV), human coronavirus (HCoVs), influenza virus (IFV), and adenovirus (HAdV) . RSV is a common cause of acute respiratory tract infections in infants and toddlers that is a cause of bronchitis and pneumonia. The aim of in this study, is to detection of RSV among children less than 5 years old hospitalized in Tabriz Childrens Hospital .
A total of 100 children less than 5 years old who had shown ARTI symptoms were recruited at the time of hospitalization from Tabriz Children’s Hospital. Viral RNA was extracted from all samples using the RNX-PLUS kit and then complementary DNA (cDNA) synthesis was performed using cDNA synthesis kit according to manufacturer’s instructions. cDNA from all samples was amplified by semi- Nested PCR technique by using the primers for N gene RSV. All amplified products were visualized on 1.5% agarose gel and the presence of a 180 base pair amplicon considered as RSV.
Of 100 enrolled children (less than 5 years old), 44 were girls and 56 were boys. No significant difference was observed in gender. The age distribution of the study population was as follows: less than 12 months (53%), 13–36 months (33%), and 37–60 months (14%). RSV recognized in 18% (18/100) in patients with acute respiratory tract infection. 10 (55.6%) of the RSV-positive samples were boys, while 8 (44.4%) were girls. Most patients (61.1%) with RSV infection were less than 12 months old, and also RSV infections had occurred mainly during winter.
According to our findings, RSV can be one of the important respiratory pathogens in young children in northwestern Iran. Consequently, implementing a routine diagnosis for respiratory pathogens can improve the management of respiratory infections in children and help combat ARTI.
RSV; ARTI; children
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