Prevalence of aac6-Ib-cr gene in clinical isolates of Klebsiella pneumoniae in Khorramabad by PCR method
نویسندگان: Fatemeh Bahrami Chegeni ©, Soheila Soleiman Nejad, Somaieh Sabzali, Pegah Shakib, Fatemeh Saleh
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Background and Aim
Klebsiella pneumoniae is one of the most important cases of community-acquired and hospital-acquired infections. This bacterium is one of the most common nosocomial pathogens. The increasing emergence of multidrug resistance among hospital isolates of Klebsiella pneumoniae has limited treatment options for treating infections caused by this bacterium. The aim of this study was to determine the frequency of aac6-Ib-cr gene in clinical isolates of Klebsiella pneumoniae.
In this study, during 7 months, people referring to laboratories in Khorramabad city were collected. The samples taken in the laboratory were evaluated differentially and biochemically and antibiotic susceptibility was evaluated by disk diffusion method. DNA extraction was performed by boiling method and genes were identified by specific primers using PCR technique.
Out of 100 samples, 63 samples (63%) were male and 37 samples (37%) were female. The highest resistance was related to cefotaxime, amikacin, tetracycline and the highest sensitivity was related to: levofloxacin, ceftazidime, ampicillin, respectively. Of the total isolates, 57 isolates had resistance, of which the prevalence of aac6-Ib-cr gene was 49.1%.
The results of this study show that the prevalence of aac6-Ib-cr gene is high and it was found that there is an increase in resistance to fluoroquinolones in the isolates.
Klebsiella pneumoniae; fluoroquinolone; antibiotic resistance; aac6-Ib-cr
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