The Genotyping of Escherichia coli O157:H7 isolated from slaughterhouses and water in different regions of Fars province using Random amplified polymorphic DNA (RAPD) analysis and Their Determining of Antibiotic Resistance.
نویسندگان: Sedigheh Mokhtary, Yahya Tahamtan ©, Mohammad Kargar
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Background and Aim
Escherichia coli O157:H7 one of the enterohemorrhagic E.coli serotypes is main food-borne pathogen that can cause severe gastrointestinal and death in human. Cattle are the major reservoir for E. coli O157:H7. During the slaughtering and production process E. coli O157:H7 enter to food chain. Identifying the source of contamination plays an important role in the control of infections. The aim of this study was to determine antibiotic susceptibility and fingerprinting of Escherichia coli O157:H7 isolated from slaughterhouses in different region of Fars province.
Twenty-three Escherichia coli O157:H7 are isolated. Random amplified polymorphic DNA (RAPD) analysis with the short primer of 1283 was used to evaluate genetic relationship among the isolates and the results was analyzed by NTSYSpc software. Antibiotic susceptibility of the isolates was examined through 7 antibiotics using the Kirby-Bauer disc diffusion method.
most isolates revealed sensitivity to seven the antibiotics and resistance rates were observed in only 3 strains with patterns ToB 10 (R)LP(R)AZM15(R) in Strain ECoRsed21, IPM10(R) in ECoRsed18 and ECoRsed22 and ToB 10 (R)LP(R) AZM 15(R) in strain ECoRsed20. DNA fingerprinting showed 9 genetic patterns for 23 strains by RAPD-PCR technique. The isolates from different sources were clustered from 31% to 100% based on similarity in RAPD pattern. The strains contain different RAPD pattern or source and had high genetic distance to others, showed different patterns of antibiotic susceptibility.
Isolates from different sources had the same genetic pattern and were located in the same cluster. Therefore, genotyping of isolates using RAPD technique is a fast, cost-effective and efficient method to determine the source of infection and its control.
Genotyping; Antibiotic Resistance; Escherichia coli O157:H7; RAPD PCR
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