The diagnostic value of β-catenin to detect invasive and non-invasive functional and non-functional pituitary adenomas
نویسندگان: Masoumeh Tavakoli-Yaraki ©, Mohammad Ghorbani
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Background and Aim
Background and aim: Pituitary adenomas as multifactorial intracranial neoplasms impose a massive burden of morbidity on patients and characterizing the molecular mechanism underlying their pathogenesis has received considerable attention. Despite the appealing role of canonical Wnt/β-catenin pathway in cancer pathogenesis, their relevance to pituitary adenoma pathogenesis and possible effect in pituitary tumor invasion is debated that is perused in the current study.
Materials and Methods: The 109 tumor tissues of non-functional, growth-hormone (GH) secreting and prolactin-secreting pituitary adenomas were obtained from patients who were subjected to the endoscopic transnasal transphenoidal surgery (ETSS) at the neurosurgery department of our institute as well as 30 cadaveric healthy pituitary tissues. The anterior epithelial lobe of pituitary with no pathological evidence was collected and the invasive pituitary tumor was determined based on the Knosp classification system. The β-catenin gene and protein level in tissues were determined using Real-Time PCR and immunohistochemistry, respectively. The receiver operating characteristic (ROC) curves and calculation of area under the curve (AUC) were used to determine the diagnostic value of β-catenin.
Results: The over-expression of β-catenin was detected in invasive compared to non-invasive functional and non-functional pituitary tumors and the β-catenin expression was associated with tumor size and invasion. Therefore, the β-catenin gene and protein expression revealed consistency in different types of pituitary tumors also the simultaneous over-expression of β-catenin in local sites of tumors and patient's PBMCs indicates the possible role of β-catenin as a diagnostic marker in pituitary tumors. The correlation of β-catenin expression with patients' age was only detected in patients with non-functional pituitary adenomas. The diagnostic value of β-catenin to distinguish non-functional pituitary adenomas from GH-secreting and prolactinoma was considerable.
Conclusion: Data from the current study provides expression pattern of β-catenin in prevalent pituitary tumors and their association with patients' clinical features which may open up new molecular targets for early diagnosis/follow up of pituitary tumor growth.
β-catenin; functional pituitary adenoma; tumor size; tumor invasion; cancer
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